Journal: Frontiers in pharmacology

Article Title: Neuroprotective Effect of Taohong Siwu Decoction on Cerebral Ischemia/Reperfusion Injury via Mitophagy-NLRP3 Inflammasome Pathway.

doi: 10.3389/fphar.2022.910217

Figure Lengend Snippet: FIGURE 5 | THSWD treatment inhibits the generationthe of ROS, activation of NLRP3 inflammasome and secretion of pro-inflammatory cytokine. (A) Quantification of ROS levels; n = 6 per group. (B–I) The Western blot relative band density of NLRP3, ASC, cleaved-caspase-1, IL-1β, IL-18; n = 6 per group. Each experiment was repeated 3 times. **p < 0.01, ***p < 0.001 versus sham group, #p < 0.05, ##p < 0.01, ###p < 0.001 versus MCAO/R group, &p < 0.05, &&p < 0.01,&&&p < 0.001 versus MCAO/R + THWSD group.

Article Snippet: Blocked with 5% nonfat milk, the membranes were incubated with the appropriate primary antibodies NLRP3 (ab263899, 1:1000, Abcam), PINK1 (af7755, 1:1000, Beyotime, China), LC3B (ab192890, 1:2000 Abcam), Parkin (ABP55480, 1:1000, abbkine, China), caspase1 (ab179515, 1:1000, Abcam), ASC (CSB-PA000940, 1:1000, CUSABIO, China), IL-1β (ABP52932, 1:750, abbkine, China) and IL-18 (ABP58918, 1:1000, abbkine, China) at 4°C overnight.

Techniques: Activation Assay, Western Blot

Journal: Acta Biochimica et Biophysica Sinica

Article Title: TXNIP aggravates cardiac fibrosis and dysfunction after myocardial infarction in mice by enhancing the TGFB1/Smad3 pathway and promoting NLRP3 inflammasome activation

doi: 10.3724/abbs.2023150

Figure Lengend Snippet: TXNIP promoted post-MI collagen deposition (A,B) Representative picrosirius red staining images of heart sections from each group at 28 days after treatment (A) under a light microscope and (B) under a polarized light microscope. Collagen I showed red/orange birefringence, while Collagen III showed green/whitish birefringence. (C,E,G) Representative immunohistochemical staining images of heart sections from each group at 28 days after treatment. (C) Collagen I deposition. (E) Collagen III deposition, and (G) ACTA2 deposition. (D) Statistical analysis of Collagen I deposition. Data are shown as the mean±SEM, n=3. **P<0.01. (F) Statistical analysis of Collagen III deposition. Data are shown as the mean± SEM, n=3. **P<0.01. (H) Statistical analysis of ACTA2 deposition. Data are shown as the mean±SEM, n=3. *P<0.05, **P<0.01.

Article Snippet: Then, the membrane was incubated with one of the following antibodies at 4°C overnight: rabbit anti-Collagen I, ACTA2, CTGF, TXNIP, Smad3, p-Smad3 (Abcam), anti-Smad7, NLRP3, ASC, Caspase-1/Cleaved Caspase-1, Mature IL1B, GAPDH (Wanleibio, Shenyang, China), mouse anti-TGFB1 antibody (Santa Cruz Biotechnology, Dallas, USA), and mouse anti-Collagen III antibody (Boster).

Techniques: Staining, Light Microscopy, Immunohistochemical staining

Journal: Acta Biochimica et Biophysica Sinica

Article Title: TXNIP aggravates cardiac fibrosis and dysfunction after myocardial infarction in mice by enhancing the TGFB1/Smad3 pathway and promoting NLRP3 inflammasome activation

doi: 10.3724/abbs.2023150

Figure Lengend Snippet: TXNIP increased post-MI collagen deposition (A) Representative images of the protein levels of Collagen III, Collagen I, ACTA2, and CTGF. (B‒E) Statistical analyses of the protein levels of Collagen I, Collagen III, ACTA2, and CTGF. Data are shown as the mean±SEM, n=3‒5. *P<0.05, **P<0.01; ns, not significant.

Article Snippet: Then, the membrane was incubated with one of the following antibodies at 4°C overnight: rabbit anti-Collagen I, ACTA2, CTGF, TXNIP, Smad3, p-Smad3 (Abcam), anti-Smad7, NLRP3, ASC, Caspase-1/Cleaved Caspase-1, Mature IL1B, GAPDH (Wanleibio, Shenyang, China), mouse anti-TGFB1 antibody (Santa Cruz Biotechnology, Dallas, USA), and mouse anti-Collagen III antibody (Boster).

Techniques: